The common solid phase extraction column is divided into three parts: medical polypropylene column tube, porous polypropylene sieve plate (20μm) and filler (mostly 40-60μm, 80-100μm) The general steps involved in performing SPE are: Pre-treat the sample (e.g. Solid phase extraction uses the difference of affinity between an analyte and interferents, present in a liquid matrix, for a solid phase (sorbent). The technically correct name for this technology is “Liquid-Solid Phase Extraction,” since the chromatographic particles are solid and the sample is in the liquid state. Solid Phase Extraction Device and Basic Operation Steps. Automated solid-phase extraction based on ASPEC™ (Gilson) technology. a. A typical solid-phase extraction cartridge consists of a short column (generally an open syringe barrel) containing a sorbent with a nominal particle size of 50–60 μm, packed between porous metal or plastic frits. About solid phase extraction cartridge:.
This affinity allows the separation of the target analyte from the interferents. A typical solid phase extraction involves four steps: (A) The four basic steps: conditioning (C), loading (L), washing (W) and eluting (E).
(B2) Eluting into the collection tubes. Solid phase extraction column (1) SPE column. The same basic chromatographic principles of liquid chromatography that are used in HPLC are also used here, but in a different format and for a different reason. (B1) Conditioning, loading and washing over the drain container. Figure 4. dilution, adjustment of pH) Condition the cartridge (run water or solvent through it) …
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